Tyrosine Hydroxylase anticorps (AA 18-133)

N° du produit ABIN968808

Cet anticorps anti-Tyrosine Hydroxylase est un anticorps Souris Monoclonal détectant Tyrosine Hydroxylase dans WB et IF. Adapté pour Rat et Souris. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour Tyrosine Hydroxylase anticorps (AA 18-133) (ABIN968808)

Antigène: Tyrosine Hydroxylase (TH)

Reactivité: Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Tyrosine Hydroxylase est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 45-Tyrosine Hydroxylase

Détail du produit anti-Tyrosine Hydroxylase anticorps

Épitope: AA 18-133

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Tyrosine Hydroxylase aa. 18-133

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Tyrosine Hydroxylase (ABIN968808)

Blanchard-Fillion, Souza, Friel, Jiang, Vrana, Sharov, Barrón, Schöneich, Quijano, Alvarez, Radi, Przedborski, Fernando, Horwitz, Ischiropoulos: "Nitration and inactivation of tyrosine hydroxylase by peroxynitrite." dans: The Journal of biological chemistry, Vol. 276, Issue 49, pp. 46017-23, (2001) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." dans: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Détails sur Tyrosine Hydroxylase

Antigène: Tyrosine Hydroxylase (TH)

Autre désignation: Tyrosine Hydroxylase

Sujet: Dopamine and its metabolic products norepinephrine and epinephrine are catecholamine neurotransmitters whose function is essential for the execution of normal neural processes in the CNS and PNS. Tyrosine hydroxylase (TH) is a non-heme iron, tetrahydrobiopterin-dependent enzyme that catalzyes the conversion of tyrosine to L-dihydroxyphenylalanine (L-DOPA). This is the rate-limiting step in the biosynthesis of catecholamines. Both the development of Parkinson’s disease and other neurodegenerative diseases result from loss of the ability to synthesize catecholamines. Decreases in the activity of TH have been implicated in these diseases. Nitration of TH at Tyrosine 423 has been associated with temporary loss of enzymatic activity, and TH nitration occurs in response to the Parkinsonian toxin MPTP, and following exposure to peroxynitrite. These findings implicate nitration as a potential mode of down-regulation of TH activity during neurodegenerative disease. Thus, TH is an essential enzyme for catecholamine synthesis, which is required for normal neuronal function.

Poids moléculaire: 58 kDa

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour